Uricase is present in numerous diverse organisms, but not in higher primates including human. Using the candida utilis urate oxidase gene sequences uricase. Uricase is an important microbial enzyme that can be used in the clinicaltreatment of gout, hyperuricemia, and tumor lysis syndrome. Mice deficient in uricase develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment. Uricase production by a recombinant hansenula polymorpha strain. The recombinant enzyme accumulates intracellularly in an active and soluble form.
The yield of recombinant uricase was significantly. Cloning, expression, and purification of recombinant. Evolutionary history and metabolic insights of ancient. Mikuls, in kelleys textbook of rheumatology ninth edition, 20. Uox is a cofactorfree homotetrameric enzyme that catalyses the conversion of uric acid to 5. Pegsiticase is a modified pegylated recombinant uricase developed to be the first. A total of 127clinical isolates of pseudomonas aeruginosa were tested for uricase production. Pegsiticase is a modified pegylated recombinant uricase developed to be the first nonimmunogenic biologic for treating the hyperuricemia of refractory gout. Uricase enzyme protein recombinant urate oxidase prospec. The enzyme urate oxidase uo, or uricase or factorindependent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5hydroxyisourate. Pegloticase commercial uricase is a recombinant uricase that can be used in humans to lower the levels of uric acid by catalyzing the oxidation of uric acid to allantoin, which is then eliminated via the kidneys. Uricase enzyme is commonly present in bacteria and fungi, but higher organisms such as humans are devoid of uricase owing to. Pegloticase is a mammalian recombinant uricase that is attached with peg polyethylene.
Cloning, expression, and purification of recombinant uricase. Recombinant urate oxidase an overview sciencedirect topics. Pegsiticase is a recombinant uricase derived from candida utilis, conjugated to peg. Genetic engineering of bacteria that can produce urate oxidase. In phase ia studies in a cohort of 22 patients, su level fell to recombinant enzyme.
Uricase is an essential enzyme that catalyzes the oxidative degradation of uric acid in the presence of oxygen to allantoin, which is a more soluble form than uric acid and can be easily excreted by the kidney 29. Gene encoding uricase enzyme is also present in human but due to some nonsense and. Pdf cloning, expression, and purification of recombinant uricase. Apseudomonas strain named ps43 showed the highest level of native uricase enzymeexpression. Packaging 250, units in glass bottle biochemphysiol actions suitable for the determination of uric acid in clinical analysis. Urate oxidase is an enzyme of the purine breakdown pathway that catalyses the oxidation of uric acid to allantoin. Pegsiticase has been examined in an openlabel, singledose 0. Thus, uricase is a promising enzyme with a high specificity toward uric acid. The open reading frame of uricase enzyme was amplified from ps43 and cloned into the expression vector prsetb. Recombinant uricase enzyme was successfully used in the treatment of hyperuricemia, with low incidence of hypersensitivity, and successfully. The uricase enzyme was firstly used as a nonrecombinant drug from aspergillus spp cultures in 1992, but it caused sensitivity and showed. Cloning and expression assessment of uricase gene from bacillus. The uricase enzyme was firstly used as a nonrecombinant drug from aspergillus spp cultures. Recombinant production of aspergillus flavus uricase and.
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